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mspi  (New England Biolabs)


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    New England Biolabs mspi
    Schematic of FLEXseq design and workflow. ( a ) The design goal and workflow of FLEXseq. The design aims to sequence adjacent regions flanking CCGG motifs while preserving the methylation markers at the motif. Information-poor regions are suppressed, while information-rich CCGG flanking regions are amplified. In the workflow, <t>input</t> <t>DNA</t> fragments are first ligated with a semi-permissive Adapter A, which serves both as an essential blocker for untargeted DNA and a required piece of targeted DNA. The nuclease <t>MspI</t> then cuts at the CCGG motif regardless of methylation status, followed by the ligation of Adapter B. Only molecules with both Adapters A and B are amplified and sequenced. ( b ) The overall workflow and analyses. Specimen inputs are sheared genomic (g)DNA from cells, cfDNA from body fluid and plasma, and fragmented DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Analyses include copy number detection for malignant aneuploidy and deconvolution of cell types.
    Mspi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 3453 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mspi/product/New England Biolabs
    Average 96 stars, based on 3453 article reviews
    mspi - by Bioz Stars, 2026-05
    96/100 stars

    Images

    1) Product Images from "Enriched methylomes of low-input and fragmented DNA using fragment ligation EXclusive methylation sequencing"

    Article Title: Enriched methylomes of low-input and fragmented DNA using fragment ligation EXclusive methylation sequencing

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkag385

    Schematic of FLEXseq design and workflow. ( a ) The design goal and workflow of FLEXseq. The design aims to sequence adjacent regions flanking CCGG motifs while preserving the methylation markers at the motif. Information-poor regions are suppressed, while information-rich CCGG flanking regions are amplified. In the workflow, input DNA fragments are first ligated with a semi-permissive Adapter A, which serves both as an essential blocker for untargeted DNA and a required piece of targeted DNA. The nuclease MspI then cuts at the CCGG motif regardless of methylation status, followed by the ligation of Adapter B. Only molecules with both Adapters A and B are amplified and sequenced. ( b ) The overall workflow and analyses. Specimen inputs are sheared genomic (g)DNA from cells, cfDNA from body fluid and plasma, and fragmented DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Analyses include copy number detection for malignant aneuploidy and deconvolution of cell types.
    Figure Legend Snippet: Schematic of FLEXseq design and workflow. ( a ) The design goal and workflow of FLEXseq. The design aims to sequence adjacent regions flanking CCGG motifs while preserving the methylation markers at the motif. Information-poor regions are suppressed, while information-rich CCGG flanking regions are amplified. In the workflow, input DNA fragments are first ligated with a semi-permissive Adapter A, which serves both as an essential blocker for untargeted DNA and a required piece of targeted DNA. The nuclease MspI then cuts at the CCGG motif regardless of methylation status, followed by the ligation of Adapter B. Only molecules with both Adapters A and B are amplified and sequenced. ( b ) The overall workflow and analyses. Specimen inputs are sheared genomic (g)DNA from cells, cfDNA from body fluid and plasma, and fragmented DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Analyses include copy number detection for malignant aneuploidy and deconvolution of cell types.

    Techniques Used: Sequencing, Preserving, Methylation, Amplification, Ligation, Clinical Proteomics, Formalin-fixed Paraffin-Embedded



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    Schematic of FLEXseq design and workflow. ( a ) The design goal and workflow of FLEXseq. The design aims to sequence adjacent regions flanking CCGG motifs while preserving the methylation markers at the motif. Information-poor regions are suppressed, while information-rich CCGG flanking regions are amplified. In the workflow, <t>input</t> <t>DNA</t> fragments are first ligated with a semi-permissive Adapter A, which serves both as an essential blocker for untargeted DNA and a required piece of targeted DNA. The nuclease <t>MspI</t> then cuts at the CCGG motif regardless of methylation status, followed by the ligation of Adapter B. Only molecules with both Adapters A and B are amplified and sequenced. ( b ) The overall workflow and analyses. Specimen inputs are sheared genomic (g)DNA from cells, cfDNA from body fluid and plasma, and fragmented DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Analyses include copy number detection for malignant aneuploidy and deconvolution of cell types.
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    Schematic of FLEXseq design and workflow. ( a ) The design goal and workflow of FLEXseq. The design aims to sequence adjacent regions flanking CCGG motifs while preserving the methylation markers at the motif. Information-poor regions are suppressed, while information-rich CCGG flanking regions are amplified. In the workflow, <t>input</t> <t>DNA</t> fragments are first ligated with a semi-permissive Adapter A, which serves both as an essential blocker for untargeted DNA and a required piece of targeted DNA. The nuclease <t>MspI</t> then cuts at the CCGG motif regardless of methylation status, followed by the ligation of Adapter B. Only molecules with both Adapters A and B are amplified and sequenced. ( b ) The overall workflow and analyses. Specimen inputs are sheared genomic (g)DNA from cells, cfDNA from body fluid and plasma, and fragmented DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Analyses include copy number detection for malignant aneuploidy and deconvolution of cell types.
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    Image Search Results


    Schematic of FLEXseq design and workflow. ( a ) The design goal and workflow of FLEXseq. The design aims to sequence adjacent regions flanking CCGG motifs while preserving the methylation markers at the motif. Information-poor regions are suppressed, while information-rich CCGG flanking regions are amplified. In the workflow, input DNA fragments are first ligated with a semi-permissive Adapter A, which serves both as an essential blocker for untargeted DNA and a required piece of targeted DNA. The nuclease MspI then cuts at the CCGG motif regardless of methylation status, followed by the ligation of Adapter B. Only molecules with both Adapters A and B are amplified and sequenced. ( b ) The overall workflow and analyses. Specimen inputs are sheared genomic (g)DNA from cells, cfDNA from body fluid and plasma, and fragmented DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Analyses include copy number detection for malignant aneuploidy and deconvolution of cell types.

    Journal: Nucleic Acids Research

    Article Title: Enriched methylomes of low-input and fragmented DNA using fragment ligation EXclusive methylation sequencing

    doi: 10.1093/nar/gkag385

    Figure Lengend Snippet: Schematic of FLEXseq design and workflow. ( a ) The design goal and workflow of FLEXseq. The design aims to sequence adjacent regions flanking CCGG motifs while preserving the methylation markers at the motif. Information-poor regions are suppressed, while information-rich CCGG flanking regions are amplified. In the workflow, input DNA fragments are first ligated with a semi-permissive Adapter A, which serves both as an essential blocker for untargeted DNA and a required piece of targeted DNA. The nuclease MspI then cuts at the CCGG motif regardless of methylation status, followed by the ligation of Adapter B. Only molecules with both Adapters A and B are amplified and sequenced. ( b ) The overall workflow and analyses. Specimen inputs are sheared genomic (g)DNA from cells, cfDNA from body fluid and plasma, and fragmented DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Analyses include copy number detection for malignant aneuploidy and deconvolution of cell types.

    Article Snippet: Sheared or unsheared genomic DNA was digested with MspI (R0106M, NEB), end-repaired, 3′-dA-tailed, and ligated to adapters using the NEBNext Ultra II modules (E7546L and E7595L, NEB).

    Techniques: Sequencing, Preserving, Methylation, Amplification, Ligation, Clinical Proteomics, Formalin-fixed Paraffin-Embedded